primary antibodies against cd34  (Affinity Biosciences)

Journal: Open Life Sciences

Article Title: Role of Embinin in the reabsorption of nucleus pulposus in lumbar disc herniation: Promotion of nucleus pulposus neovascularization and apoptosis of nucleus pulposus cells

doi: 10.1515/biol-2022-0878

Figure Lengend Snippet: Embinin promotes lumbar disc neovascularization in LDH rats. (a) H&E staining showed the nucleus pulposus tissues of rats upon the indicated treatment. Significant morphological changes and degeneration of the nucleus pulposus were observed in the LDH group, and the amount of NP within the disc was dramatically decreased after surgery. Scale bar, 200 μm. (b) IHC assays showed the expression of CD34 in the nucleus pulposus tissues of rats upon the indicated treatment. The percentage of CD34-positive cells were calculated. Scale bar, 200 μm. The arrows indicate the positive signal. (c) ELISA assays showed the secretion of VEGF in the nucleus pulposus tissues of rats upon the indicated treatment. Error bars indicate SD. *** p < 0.001, LDH vs sham, ^^ p < 0.01, ^^^ p < 0.001, LDH + Embinin vs LDH.

Article Snippet: Primary antibody against CD34 (ab81289, 1:500, Abcam, Cambridge, UK) was incubated with the sections at 4°C overnight.

Techniques: Staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Neuroanatomy

Article Title: Arterial supply and morphological characteristics of sympathetic neurons in the human superior cervical ganglion

doi: 10.3389/fnana.2024.1372180

Figure Lengend Snippet: Arterial supply and micromorphological characteristics of the superior cervical sympathetic ganglion (SCSG). (A) SCSG (1) with borders indicated by small arrows receives three branches (large arrows) coming from the ascending pharyngeal artery (2), posterior to the internal carotid artery (3), vagus nerve (4) and internal jugular vein (5); posterior pharyngeal wall (6); occipital condyle, transected (7) (view from the back after disarticulation of the head and reflection of the pharyngeal wall anteriorly from the vertebral column). (B) SCSG specimen stained with hematoxilin and eosin (H&E) stain. (C) SCSG specimen stained with the Masson trichrome method. (D) SCSG specimen stained with silver staining method. (E) SCSG specimen and (F) small-magnification view showing vascular supply of the ganglionic tissue (CD34 immunostaining). (G) SCSG specimen and (H) small-magnification view showing mast cells related to the ganglionic tissue (mast cell tryptase immunostaining).

Article Snippet: The slices underwent the immunostaining by the incubation with the following mouse monoclonal primary antibodies; against CD34 (DAKO A/S, Denmark M 7165, 1:25), and anti-mast cell tryptase (DAKO A/S, Denmark M 7052, 1:100).

Techniques: Staining, Silver Staining, Immunostaining

Journal: Frontiers in Neuroanatomy

Article Title: Arterial supply and morphological characteristics of sympathetic neurons in the human superior cervical ganglion

doi: 10.3389/fnana.2024.1372180

Figure Lengend Snippet: (A–C) Immunohistochemical characteristics of the superior cervical sympathetic ganglion (SCSG) blood supply (CD34 immunostaining), and (E–H) mast cells related to the human SCSG neurons (mast cell tryptase immunostaining). (A) Slender network of intraganglionic microvessels surrounding the ganglionic neurons of SCSG and parallel microvessels within the fascicle of axons and fibrous tissue. (B) Delicate plexiform microvessels of the upper part of SCSG fill the interneuronal spaces. (C) Dense network of microvessels of the lower part of SCSG covering the satellite glial cells and the deeper neurons. (D) Detail of higher magnification of the ganglionic capillary plexus injected with India ink and cleared in methyl salicylate. (E) Low-magnification of the upper part of SCSG specimen and (F) high-magnification view showing mast cells (arrows) related to the neurons (mast cell tryptase immunostaining). (G) Low-magnification of the lower part of SCSG specimen and (H) high-magnification view showing mast cells (arrows) related to the ganglionic tissue (mast cell tryptase immunostaining). (I) Tryptase-positive mast cells (yellow arrows) always in close vicinity to blood vessels (red arrows). (J) Numerous mast cells (yellow arrows) found between the axons of the internal carotid nerve at the exit from SCSG.

Article Snippet: The slices underwent the immunostaining by the incubation with the following mouse monoclonal primary antibodies; against CD34 (DAKO A/S, Denmark M 7165, 1:25), and anti-mast cell tryptase (DAKO A/S, Denmark M 7052, 1:100).

Techniques: Immunohistochemical staining, Immunostaining, Injection

Journal: World Journal of Stem Cells

Article Title: Silencing of Jumonji domain-containing 1C inhibits the osteogenic differentiation of bone marrow mesenchymal stem cells via nuclear factor-κB signaling

doi: 10.4252/wjsc.v16.i2.151

Figure Lengend Snippet: Osteogenic and adipogenic differentiation of bone mesenchymal stem cells. A: Bone mesenchymal stem cells (BMSCs) were isolated from mice bone marrow tissues. Flow cytometry was used to detect the expressions of CD29, CD31, CD90, CD45, CD34, MHCII, SCA-1 and CD11b; B-D: BMSCs were induced towards osteogenic differentiation. ALP staining was used to evaluate alkaline phosphatase (ALP) activity (B); calcium deposits were visualized by Alizarin red staining (C); the mRNA levels of osteogenic genes, including Osteocalin, Runt-related transcription factor 2 and ALP, were detected by reverse transcription coupled to the quantitative polymerase chain reaction (RT-PCR) (D); E and F: BMSCs were induced towards adipogenic differentiation. Representative images of Oil red O staining (E), the mRNA levels of adipogenic genes, such as peroxisome proliferator-activated receptor gamma and CCAAT enhancer-binding protein alpha, were measured by RT-PCR (F). All values are shown as mean ± SD. d P < 0.0001. n = 3. BMSC: Bone mesenchymal stem cell; ALP: Alkaline phosphatase; RunX2: Runt-related transcription factor 2; CEBPα: CCAAT enhancer-binding protein alpha; PPARγ: Peroxisome proliferator-activated receptor gamma.

Article Snippet: After centrifugation at 1500 rpm, the cells were incubated for 0.5 h with primary antibodies against CD29, CD31, CD34, CD45, CD90, MHCII, SCA-1 and CD11b (Biolegend, California, San Diego, United States and Becton Dickinson, Franklin Lakes, NJ, United States).

Techniques: Isolation, Flow Cytometry, Staining, Activity Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Binding Assay